Jesse Lingard, Ander Herrera, Ashley Young, and Marcos Rojo missed Manchester United training ahead Champions League home game against Valencia on Tuesday.
Lingard and Rojo missed the 3-1 defeat to West Ham on Saturday due to an injury.
Young played the match against West Ham in which Herrera was an unused substitute, but both are doubts for Tuesday.
Antonio Valencia also missed the trip to the London Stadium but took part in the session at Carrington on Monday.
United who are currently top of their Champions League group after a 3-0 win over Young Boys in their first game face Valencia on the back of three games without a win.
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